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primers 8334  (Addgene inc)


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    Structured Review

    Addgene inc primers 8334
    Primers 8334, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primers+8334/pgex-cIAP1+mut+(Plasmid+%238334)/ppr0066612-67-35-19
    Average 90 stars, based on 1 article reviews
    primers 8334 - by Bioz Stars, 2026-10
    90/100 stars

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    Plasmid Preparation:

    Article Title: Evolutionarily conserved roles of Caenorhabditis elegans perilipin in lipolysis
    Article Snippet: The repair plasmid was prepared by four-fragment Gibbson 119 Assembly (Gibson Assembly® Mix, New England Biolabs, Ipswich, Massachusetts, USA) of a 120 579bp-long upstream and a 585bp-long downstream homologous flanking regions PCR121 amplified with the primers 8342 and 8343; and 8344 and 8345 respectively, which had 5’- 122 overhangs homologous to remaining fragments. .. The mKate2 fluorescent protein with the SEC as 123 well as the vector backbone were obtained from pDD285 plasmid (Addgene plasmid # 66826) 124 (Dickinson et al. 2015) by PCR amplification with the use of primers 8334, 8335, 8336 and 8337 125 (Table S1, Fig. S2). .. Both plasmids were microinjected into gonads of young adult N2 126 hermaphrodites as described (Tabara et al. 2017; Vohanka et al. 2010) using an Olympus IX70 127 microscope equipped with a Narishige microinjection system (Olympus, Tokyo, Japan) along 128 with red fluorescent co-injection markers pCFJ104 [Pmyo-3::mCherry::unc-54] and pGH8 129 [pRAB-3::mCherry::unc-54utr] (Addgene plasmids # 19328 and # 19359 respectively) (Frøkjær130 Jensen et al. 2008).

    Polymerase Chain Reaction:

    Article Title: Evolutionarily conserved roles of Caenorhabditis elegans perilipin in lipolysis
    Article Snippet: The repair plasmid was prepared by four-fragment Gibbson 119 Assembly (Gibson Assembly® Mix, New England Biolabs, Ipswich, Massachusetts, USA) of a 120 579bp-long upstream and a 585bp-long downstream homologous flanking regions PCR121 amplified with the primers 8342 and 8343; and 8344 and 8345 respectively, which had 5’- 122 overhangs homologous to remaining fragments. .. The mKate2 fluorescent protein with the SEC as 123 well as the vector backbone were obtained from pDD285 plasmid (Addgene plasmid # 66826) 124 (Dickinson et al. 2015) by PCR amplification with the use of primers 8334, 8335, 8336 and 8337 125 (Table S1, Fig. S2). .. Both plasmids were microinjected into gonads of young adult N2 126 hermaphrodites as described (Tabara et al. 2017; Vohanka et al. 2010) using an Olympus IX70 127 microscope equipped with a Narishige microinjection system (Olympus, Tokyo, Japan) along 128 with red fluorescent co-injection markers pCFJ104 [Pmyo-3::mCherry::unc-54] and pGH8 129 [pRAB-3::mCherry::unc-54utr] (Addgene plasmids # 19328 and # 19359 respectively) (Frøkjær130 Jensen et al. 2008).

    Amplification:

    Article Title: Evolutionarily conserved roles of Caenorhabditis elegans perilipin in lipolysis
    Article Snippet: The repair plasmid was prepared by four-fragment Gibbson 119 Assembly (Gibson Assembly® Mix, New England Biolabs, Ipswich, Massachusetts, USA) of a 120 579bp-long upstream and a 585bp-long downstream homologous flanking regions PCR121 amplified with the primers 8342 and 8343; and 8344 and 8345 respectively, which had 5’- 122 overhangs homologous to remaining fragments. .. The mKate2 fluorescent protein with the SEC as 123 well as the vector backbone were obtained from pDD285 plasmid (Addgene plasmid # 66826) 124 (Dickinson et al. 2015) by PCR amplification with the use of primers 8334, 8335, 8336 and 8337 125 (Table S1, Fig. S2). .. Both plasmids were microinjected into gonads of young adult N2 126 hermaphrodites as described (Tabara et al. 2017; Vohanka et al. 2010) using an Olympus IX70 127 microscope equipped with a Narishige microinjection system (Olympus, Tokyo, Japan) along 128 with red fluorescent co-injection markers pCFJ104 [Pmyo-3::mCherry::unc-54] and pGH8 129 [pRAB-3::mCherry::unc-54utr] (Addgene plasmids # 19328 and # 19359 respectively) (Frøkjær130 Jensen et al. 2008).



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    primers 8334  (Addgene inc)
    90
    Addgene inc primers 8334
    Primers 8334, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primers+8334/pgex-cIAP1+mut+(Plasmid+%238334)/ppr0066612-67-35-19
    Average 90 stars, based on 1 article reviews
    primers 8334 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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